化学生物学论文英文译文

学 位： 博士Email：职 称：教师简介：英语学院教授,为本科生开设汉英笔译、应用文翻译和为翻译方向研究生开设学术论文写作课和汉英文化比较与翻译 2008年7月到8月于英国兰开斯特大学作短期访学； 2005年1月 至2005年12月在英国爱丁堡大学文学、语言和文化学院从事博士后研究，并给亚洲学院中文系的本科生讲授中国文化。研究课题：研究工作围绕着翻译教学与理论研究展开，包括翻译理论与实践，西方当代翻译理论，实务翻译与教学。当前从事的研究课题为北京外国语大学211三期立项项目“汉译英翻译能力研究”、商务翻译理论与实践出 版 物：自1999年以来已经发表专著一部，教程两部，有独到见解的论文二十余篇，多数发表于核心刊物、或SSCI, CSSCI期刊以及国际学术期刊BABEL 、META。

这样：Biosensors in food, medicine, environmental testing and many other fields have a wide range of applications, in recent years, the development of increasingly One of many biosensor for immunization on clinical research, it can significantly shorten the testing time, and easy to operate and difficult to The main thesis of this study the clinical diagnosis of hepatitis B virus immunosensor preparation, the use of sparse matrix (-SH) can form a stable with the Au-S bond, fixed at first thiourea on gold electrode, and then the use of cross-linking agent glyoxal hepatitis B antibody (HBsAb) fixed on the electrode to form the monolayer, made for the detection of hepatitis B surface antigen (HbsAg) Immunosensor Through cyclic voltammetry to examine the electrochemical properties of The sensor produced a simple, convenient operation, short response time, high sensitivity, can be used to detect hepatitis B surface Studies have shown that the sensor detection range of 5-200ug / L, detection limit is 1 ug / L

原文： 9-[2-(3-Carboxy-9,10-diphenyl)anthryl]-6-hydroxy-3H-xanthen-3-ones (DPAXs) The most widely used 1O2 trap is 9,10-diphenylanthracene (DPA), which reacts rapidly and specifically with 1O2 to form a thermostable endoperoxide at a rate of k =3106 M1 The decrease in absorbance at 355 nm is used as a measure of the formation of the However, DPA derivatives are not very sensitive probes because the detection is based on the measurement of absorbance [79] Umezaka et [79] fused DPA with a fluorophore (fluorescein) aiming to associate the first’s reactive characteristics with the second’s fluorescent Fluorescein was chosen as fluorophore since it has a high fluorescence quantum yield in aqueous solution and is able to be excited at long From this fusion resulted 9-[2-(3-carboxy-9,10-diphenyl)anthryl]-6- hydroxy-3H-xanthen-3-ones (DPAXs) (F 11) [79] Thus, DPAXs were the first chemical traps for 1O2 that permitted fluorescence They react with 1O2 to produce DPAX endoperoxides (DPAX-EPs) (F 11) DPAXs themselves scarcely fluoresce, while DPAXEPs are strongly The mechanism accounting for the diminution of fluorescence in DPAXs and its enhancement in DPAX-EPs remain unclear [79] The fluorescence intensity of fluorescein derivatives is known to be decreased under acidic conditions as a consequence of the protonation of the phenoxide oxygen In order to stabilize the fluorescence intensity at physiological pH, electron-withdrawing groups wereincorporated at the 2- and 7-positions of the xanthene chromophore, leading to Cl (DPAX-2) and F (DPAX-3) (F 11) This modification lowered the pKa value of the phenolic oxygen atom [79] DPAX-2 was used to detect the production of 1O2 from two different generation systems: the MoO4 2/H2O2 system and the 3-(4-methyl-1-naphthy)propionic acid endoperoxide (EP-1) system, which act at different pH values (5 and 4, respectively) In both cases an increase of the probe’s fluorescence was verified when in contact with the generating These results confirmed DPAXs’ advantage when detecting 1O2 in neutral or basic aqueous solutions [79] The behaviour of this probe towards H2O2, !NO and O2 ! was also studied, but no change in the intensity of the fluorescence was observed for any of these reactive These facts corroborate the specificity of this probe for 1O2 [79] The detection of 1O2 in biological samples was also With this purpose, DPAX-2 diacetate (DPAX-2-DA) was prepared, since it was considered to be more permeable to DPAX-2-DA is hydrolysed by intracellular esterases to generate DPAX- Both DPAX-2 and DPAX-2DA were tested and compared in the same assay However, cells were stained similarly in both This observation probably means that DPAX-2 itself is also membranepermeable [79]译文： 91-[2-(3-羧基-9,10-二苯)anthryl]-6 羟基3h-xanthen-3-酮 (dpaxs)使用最广泛的1o2阱9,10-diphenylanthracene(政治部) 而迅速的反应,特别是与1o2形成耐热endoperoxide的增长率为k=3106米1秒 减少6%,为355nm,是用来衡量形成的过氧化物 不过,政治部衍生不是很敏感的探针,因为检测是基于测量吸光度[79] [79]fused审批与fluorophore(fluorescein)以准第一功的特点与第二的荧光特性 fluorescein被选为fluorophore,因为它具有较高的荧光量子产率在水溶液中,并能 兴奋长波长 由此导致的融合 91-[2-(3-羧基-9,10-二苯)anthryl]-6 - 羟基3h-xanthen-3酮(dpaxs)(图11)[79] 因此,dpaxs被化学第一陷阱1o2允许荧光检测 他们的反应与1o2出示dpaxendoperoxides(dpax-eps)(图11) dpaxs自己scarcelyfluoresce,而dpaxeps强烈的荧光 机制的会计核算窄化荧光dpaxs及其增强dpax-办事仍不清楚[79] 荧光强度的荧光素衍生物已知是减少酸性条件下,随着大量的质子 该phenoxide氧原子 为了稳定的荧光强度,在生理pH, 电子撤组wereincorporated在2-和7点位置的xanthene生色 通往cl(dpax-2)和F(dpax-3)(图11) 这个修改降低pKa值的酚氧原子〔79〕 dpax-2检测生产1o2从两个不同的发电系统: 该moo42/过氧化氢体系和3-(4-甲基-1-萘基)丙酸endoperoxide(ep-1)系统, 该法在不同pH值(5和4美元) 在这两种情况下,提高了探头的荧光验证时接触的发电系统 这些调查结果证实dpaxs的优势,在检测1o2在中性或碱性溶液[79] 该行为此探针对双氧水,! NO和O2! 还研究, 但不改变强度的荧光染色任何这些活性物种 这些事实证实特异性这种探针1o2[79] 检测1o2生物样品中为75% 为了这个目的,dpax-2diacetate(dpax-2-da)制成的,因为它被认为是更容易接受细胞 dpax-2-daishydrolysedbyintracellularesterases产生dpax- 双方dpax-2dpax-2da试验,比较在同一实验系统 但是,细胞染色同样,在这两种情况 这个观察可能意味着dpax-2本身也是membranepermeable[79]